Inclined Single Plane Illumination Microscopy (ISPIM)
نویسندگان
چکیده
منابع مشابه
Inverted selective plane illumination microscopy (iSPIM) enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis elegans.
The Caenorhabditis elegans embryo is a powerful model for studying neural development, but conventional imaging methods are either too slow or phototoxic to take full advantage of this system. To solve these problems, we developed an inverted selective plane illumination microscopy (iSPIM) module for noninvasive high-speed volumetric imaging of living samples. iSPIM is designed as a straightfor...
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Long-lived, photoinduced transient dark states of fluorophores have a great potential for environmental sensing. Transient State (TRAST) Imaging based on time-modulated excitation has recently been shown to provide imaging of the kinetics and populations of these states in biological samples and has been realized for confocal, wide-field and total internal reflection microscopy settings. In thi...
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Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high d...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.1048